Easy and fast in vitro Transcription/Translation in one tube without cell culture.
The Genelator™ in vitro Transcription/Translation Kit uses highly productive E. coli lysates, which contain all transcriptional and translational machinery components (chaperones, ribosomes, ribosomal factors, tRNAs, aminoacyl-tRNA synthesis, etc.) as well as T7 RNA polymerase. It is a coupled transcription- translation system that can be used to express full-length proteins from T7 or E.coli promoters in a single-step reaction using supercoiled (plasmid) DNA templates. Using the Genelator™ Kit, up to 600ug/ml biologically active protein can be synthesized within 60minutes. The synthesis reaction can be easily scaled up (to produce mg amount of protein synthesized increases linearly with increased reaction volume).
The Genelator™in vitro Transcription/Translation Kit is more suitable for the enhanced soluble production of aggregation-prone protein compared to conventional cell-free protein synthesis. Genelator™ Kit is prepared from genetically engineered E.coli strains which are modified to have enhanced levels of folding-related factors as a folding aid for better solubility of proteins. Genelator™ Kit serves as a powerful technology for the production of aggregation-sensitive proteins originated from eukaryotes.
We expect this Genelator™ Kit will be helpful in production of a higher level of soluble proteins for further biochemical analysis and proteomics research such as structural genomics and functional genomics. And it is applicable to preparation of protein samples required in the actively growing research areas such as structural genomics, functional genomics, protein chip, diagnosis, protein engineering, bioelectronics including biosensor, and lab-on-a-chip.
0 / 4000
At least 10~1000 characters.