Newcastle Disease Virus - common & patho Detection Kit
Newcastle Disease Virus - common & patho Detection Kit is direct detection of Avian mycoplasmosis(M. synoviae and M. gallisepticum) on the basis of a genetic database.
Fast, accurate and reliable diagnosis
- It takes only 2~3 hours for detection.
- It can amplify only specific gene using the RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method.
Newcastle disease is caused by an RNA virus, paramyxovirus-1 (PMV-1), that can be categorized into three groups: the velogenic strains, which are highly pathogenic and easily transmitted; the mesogenic strains, which show intermediate pathogenicity; and the lentogenic strains, which show low pathogenicity in chickens.The signs depend greatly on whether the virus is neurotropic or viscerotropic. The neurotropic viruses result in respiratory and nervous signs. The viscerotropic viruses, which predominate worldwide, cause respiratory signs with peracute disease, watery-greenish diarrhea, and swelling of the tissues of the head and neck. Nervous signs are also frequently seen in exotic bird species (parrots, etc) infected with viscerotropic viruses and in poultry species that survive beyond the acute phase of the disease. Newcastle disease virus -common & patho Detection Kit is direct detection of newcastle diseasevirus on the basis of a genetic database,so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 2~3 hours for detection. Therefore, it is a very fast accurate, reliable technique.
- Expected NDV PCR product size : 379bp
- Expected Pathogenic NDV PCR product size : 204bp
Fig 1. Result of New castle disease detection with 10-fold diluted allantoic fluid.
- The allantoic fluid was 10-fold diluted 108.0 EID50/0.1ml, then RNA isolated and performed the RT-PCR by Newcastle disease virus - common & patho Detection Kit.
- Caecal tonsil
- Bronchial swab
- Bronchi-lungs-air sacs
The specimen should be stored at -20°C prior to use
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