Biotin is used in two-step detection systems in concert with conjugated avidin.
Biotin is typically conjugated to proteins via primary amines (i.e., lysines).
Usually, between 3 and 6 biotin molecules are conjugated to each antibody.
The entire conjugation can be performed in about an hour.
In general, you will need to have a solution of your antibody at a concentration (optimally) of at least 2 mg/ml. The extent of biotin conjugation to the antibody may depend on the concentration of antibody in solution; for consistent conjugations, use a consistent concentration.
This product provides convenient and efficient method for removing salt and amine compound interfering biotin coupling reaction from antibody solution.