Molecular test is one of the most effective way to diagnose the presence of 2019- nCoV in people who are thought to be infected. In particular, real-time reverse-transcription polymerase chain reaction (rt RT-PCR) assays are molecular tests that can be used to detect viral RNA in clinical samples. WHO’s current case recommendation for laboratory confirmation of 2019-nCoV infection requires either a positive rt RT-PCR result for at least two specific genomic targets (RdRp and E-gene) and a single positive target. OPTOLANE Technologies Inc. introduces The Kaira 2019-nCoV Detection Kit to diagnose 2019-nCoV in human samples through reverse transcription Real-Time polymerase chain reaction (rt RT-PCR).The Kaira 2019-nCoV Detection Kit is designed for qualitative detection of COVID-19 Virus (SARS-CoV-2) RNA in the specimens of suspected respiratory disease patients (sputum, oral swab, nasal swab) in in vitro settings, by Real-Time Polymerase Chain Reaction (RT-PCR), assisting diagnosis of COVID-19. For professional use only.
(Storage: reagent: Within 12 months, -20 ℃, anaylzer Shipment: reagent-cold chain)
Real-time PCR involves the selective amplification of a target sequence while monitoring the progress of amplification in real-time through a visualizing agent such as a fluorescent dye. The specificity is provided by a pair of specific primers, along with a hydrolysis probe which is also sequence specific. Monitoring amplified product is conducted by labeling the hydrolysis probe with a matched pair of fluorescent dyes (5’-Fluorescent reporter; 3’- Quencher). However, upon cleavage by the 5’ – 3’ exonuclease activity of the DNA polymerase during PCR, the fluorescent reporter molecule will emit a specific wavelength of light within the visible spectrum when cleaved after binding to the amplicon.